Example technologies where Randall J Brezski was an inventor or co-inventor
Protease resistant antibodies
Fc engineered antibodies with selective engagement of Fc gamma receptors
Liver specific Wnt signal enhancing molecules
Vhh-IgG fusion protein Wnt pathway agonists
Antigen-binding complexes having enhanced agonist activity
Humanized antibodies
Antibody VH/VL sequences against numerous targets
IgG-centyrin fusion proteins (mAbtyrin) targeting S. aureus and bicomponent leukotoxins
Adapted from Wang, Mathieu, and Brezski. IgG Fc engineering to modulate antibody effector functions.
Select publications
Wang, X., M. Mathieu, R.J. Brezski. 2018. IgG Fc engineering to modulate antibody effector functions. Protein Cell. 9(1):63-73.
Huang, T., M. Mathieu, S. Lee, X. Wang, Y.S. Kee, J.J. Bevers 3rd, C. Ciferri, A. Estavez, M. Wong, N.Y. Chiang, G. Nakamura, R.J. Brezski. 2018. Molecular characterization of human anti-hinge antibodies derived from single cell cloning of normal human B cells. J Biol Chem. 293(3):906-919.
Lo, M., H.S. Kim, T.W. Bainbridge, J.M. Vernes, Y. Zhang, Y.L. Lin, S. Chung, M.S. Dennis, Y.J. Zuchero, R.J. Watts, J.A. Couch, Y.G. Meng, J.K. Atwal, R.J. Brezski, C. Spiess, J.A. Ernst. 2017. Effector attenuating substitutions that maintain antibody stability and reduce toxicity in mice. J Biol Chem. 292(9):39000-3908.
Moores, S., M. Chiu, B. Bushey, K. Chevalier, L. Luistro, K. Dorn, R.J. Brezski, P. Haytko, T. Kelly, S. Wu, J. Neijssen, P. Parren, J. Schuurman, R. Attar, S. Laquerre, M.V. Lorenzi, G.M. Anderson. 2016. A novel bispecific antibody targeting EFGR and cMet that is effective against EGFR inhibitor-resistant lung tumors. Cancer Research. 76(13):3942-3953.
Brezski, R.J., G. Georgiou. 2016. Immunoglobulin isotype knowledge and application to antibody engineering. Current Opinion in Immunology. 40:62-69.
Kinder, M., A.R. Greenplate, W.R. Strohl, R.E. Jordan, R.J. Brezski. 2015. An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions. mAbs. 7(3):494-504.
Brezski R.J., M. Kinder, K.D. Grugan, K.L. Soring, J. Carton, A.R. Greenplate, T. Petley, D. Capaldi, K. Brosnan, E. Emmell, S. Watson, R.E. Jordan. 2014. A monoclonal antibody against hinge-cleaved IgG restores effector function to proteolytically-inactivated IgGs in vitro and in vivo. mAbs. 6(5):1265.
Kinder, M., A. R. Greenplate, K. D. Grugan, K. L. Soring, K. A. Heeringa, S. G. McCarthy, G. Bannish, M. Perpetua, F. Lynch, R. E. Jordan, W. R. Strohl, R. J. Brezski*. 2013. Engineered protease-resistant antibodies with selectable cell-killing functions. J Biol Chem. 288(43):30843-54.
Vafa, M., G.L. Gilliland, R.J. Brezski, B. Strake, T. Wilkinson, E.R. Lacy, B. Scallon, A. Teplyakov, T. Malia, W.R. Strohl. 2013. An engineered silent Fc variant of an IgG eliminates all immune effector functions via structural perturbations. Methods. S1046-2023(13)00247-8.
Grugan, K.D., F.L. McCabe, M. Kinder, A. Oberholtzer, B.C. Harman, J. Ekert, N. Rooijen, G.M. Anderson, J. Nemeth, W.R. Strohl, R.E. Jordan, R.J. Brezski. 2012. Tumor-associated macrophages promote invasion while retaining Fc-dependent anti-tumor function. J Immunol. 189(11):5457-5466.
Brezski, R.J. and R.E. Jordan. 2010. Cleavage of IgGs by proteases associated with invasive diseases: an evasion tactic against host immunity? mAbs. 2(3):212-220.
Brezski, R.J., O. Vafa., D. Petrone, S.H. Tam, G. Powers, M.H. Ryan, J.L. Luongo, A. Oberholtzer, D.M. Knight, R.E. Jordan*. 2009. Tumor-associated and microbial proteases compromise host IgG effector functions by a single cleavage proximal to the hinge. PNAS. 106(2):17864-9.
Brezski, R.J. and J.G. Monroe. 2007. BCR-induced Rac1 activation and Rac1-dependent spreading are impaired in transitional immature B cells due to levels of membrane cholesterol. J Immunol. 179(7):4464.
Issued patents
Yang. L., Z. Zhang, R. Brezski, L. Presta, T. Lopez, H. Chen, H. Baribault, W.C. Yeh, S. Tu. Liver-specific Wnt signal enhancing molecules and uses thereof. Patent No. 12,240,876. Patent Date. 3/4/2025.
Yang, L., T.Z. Yuan, A.K. Sato, W.C. Yeh, C.Y. Janda, T.W. Fowler, H. Baribault, K.P. Lai, L. Xie, R.J. Brezski, C. Lu. Wnt surrogate molecules and uses thereof. Patent No. 11,773,171. Patent Date. 10/3/2023.
Lazar, G., J. Kim, J. Zhu, Y. Yang, R. Brezski. Antigen binding complex having agonistic activity and methods of use. Patent No. 10,865,248. Patent Date. 12/15/2020.
Brezski, R.J., A.S. Lynch, P.T. Buckley, J. Fernandez, J. Luo, T.J. Malia, S.J. Wu, V.J. Torres. Compositions and methods for anti-staphylococcal biologic agents. Patent No. 10,781,246. Patent Date. 9/22/2020.
Simons, P.J., L. Boon, J. Luo, R. Brezski, M. Goldberg. Humanized anti-CD134 (OX40) antibodies and uses thereof. Patent No. 10,273,307. Patent Date. 4/30/2019.
Torres, V.J., R.J. Brezski, A. Lynch, W. Strohl, B. Whitaker, M. Chui, P.T. Buckley, K. Dorn, M. Kinder. Compositions and methods for phagocytic delivery of anti-staphylococcal agents. Patent No. 10,087,243. Patent Date. 10/2/2018.
Simons, P.J., L. Boon, J. Luo, R. Brezski, M. Goldberg. Humanized anti-CD134 (OX40) antibodies and uses thereof. Patent No. 9,790,281 B2. Patent Date 10/17/2017.
Torres, V.J., R.J. Brezski, A. Lynch, W. Strohl, B. Whitaker, M. Chui, P.T. Buckley, K. Dorn, M. Kinder. Compositions and methods for phagocytic delivery of anti-staphylococcal agents. Patent No. 9,644,023 B2. Patent Date 05/09/2017.
Brezski, R., R. Jordan, W. Strohl. Active protease-resistant antibody Fc mutants. Patent No. 9,611,328 B2. Patent Date 04/04/2017.
Jordan, R., D. Knight, R. Brezski, M. Ryan, D. Petrone. Immunoglobulin cleavage fragments and disease indicators and compositions for detecting and binding such. Patent No. 9,481,734 B2. Patent Date 11/01/2016.
Brezski, R., R. Jordan, W. Strohl. 2014. Active protease-resistant antibody Fc mutants. Patent No. 8,871,204 B2. Patent Date 10/28/2014.
Jordan, R., D. Knight, R. Brezski, M. Ryan, D. Petrone. Immunoglobulin cleavage fragments as disease indicators and compositions for detecting and binding such. Patent No. 8,501,907 B2. Patent Date 08/06/2013.